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WATSON BIOLAB >  Products >  LeukoCatchⅡ

LeukoCatchⅡ for removal of Leukocyte

Leuko filter traps leukocytes specifically from a whole blood.

Easy-to-Use Column Type

LeukoCatch2

Cat. No. Item Packaging
1883-110LC LeukoCatchⅡ Column Type 5pcs.

leuko2_howto

The leukocyte count after filtration by LeukoCatch Ⅱ

6 out of 20 fields randomly obtained from the microscopic observation of a mouse blood.

*Data provider : Mie University, Faculty of Medicine, The Laboratory of Medical Zoology

■before filtration

80cells (in the whole 20 fields)

■after filtration with Leuko Catch II

5cells (in the whole 20 fields)

 

LeukoCatchⅡ Q&A

Is it possible to use a whole blood as it is without dilution?
It is possible but filtration time is much longer. Also it causes clog on the porous filter much quicker, thus operation efficincy may drop. It is suggested that a whole blood be diluted 2-3 fold with PBS.
Is it possible to collect leukocytes with LeukoCatchⅡ?
A protocol for that purpose has not been established at this moment and it is under development.
Is it possible to use a animal blood?
A blood from different animals may cause difference in the results. Please try with a sample product first. Our in-house evaluation is conducted with human and mouse bloods.
Is there any tendency in the efficiency of leukocyte removal due to the types or sizes of a leukocyte ?
Basically, the types or sizes do not affect the efficiency and uniform results can be obtained. There is not a case where, for example, only limphecytes are left unremoved.
 

Note: As cited from an article in Advances in Bioscience and Biotchnology, 2012.
“Nevertheless, these leukocyte separation procedures require laboratory-based professional and time-consuming procedures using instrumentation that is not routinely available at the bedside or in a common clinical facility. Moreover, their protocols are too complex to apply to an automated machine to treat hundreds of samples simultaneously.”

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