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Preservation Plate

New form of bioresource storage

Watson's Preservation Plate (PVP) has been developed in order to enable storage and transportation of such bioresources as nucleic acids (DNA / RNA / oligonucleotide) and blood under room temperature and pressure.
Simply place such samples onto paper chips, then dry, the preservation is completed.

Space Saving

It is very compact and space saving in comparison with preserving in tubes samples of liquid condition. Marking space on the plate and compact body make sample storage management easier.

Recover Samples Directly on PCR Plate

Paper chips can be put directly into the solution to start PCR or in situ hybridization.

Reduce Contamination Risk

Compared to the traditional way of repeated samplings from the same tube, Preservation Plate (PVP) can reduce contamination risk by using different wells on the PVP and/or changing the PVP sheet itself dependent on the sample (Note: Only 96well PVP can be cut easily with scissors).

Prevent Sample Deterioration

Sample deterioration is prevented as the PVP requires no repetition of freezing and melting.

No Reagent or Salt Used

Paper chips do not contain any reagents or salt so it does not choose type of eluent to be used when you recover samples.

Rich Selection

Please find a suitable plate type for different bioresourses and sample volumes.

【Sample Volume】
  • 5μL/sample   ⇒ 96Well
  • 50μL/sample  ⇒ 3Well
  • 200μL/sample ⇒ 1Well
【Sample type】
  • Nucleic Acid
    (DNA, RNA, oligonucleotide, plasmid,  RNA probe, genomic DNA etc.)
     ⇒ Nylon paper chip
  • Blood, throat swab, feces etc.
     ⇒ Cellulose paper chip

How to Use

 
Preservation Method

①Let the paper chip absorb a liquid sample.

A: For a liquid sample
B: For a sample from agar

* Protective agent is spread on paper chips and it may appear as dot pattern but it does not affect the qualities.

②Dry the sample.

*Reduced pressure drying is recommended.

Recommended dry time
[96 well] 60 minutes or more
[1/3 well] 90 minutes or more

* Insufficient drying may result in faulty performance.

③Seal the plate with the protection seal and store it in room temperature.

* Make sure that the film is tightly applied. Loose sealing may cause contamination.

Recovery Method

①Peel off the protection seal and place the paper chip into a container.

Cut out the necessary size of paper chip.

Pick up the paper chip from tweezer hole.

Push the paper chips by tweezers etc.

②Put the solution into the container and stir. In the case of agar, rub the paper chip onto agar.

 
 

Lineup

96well

To the level 5μL of solution volume per well

  • Dramatic savings per chip!
  • Made from Polypropylene -
    it is easy to cut!
  • Convenient well layout to match your 96 well plate!

caplock01 Lower cost per well.

caplock01 Easy to cut

caplock01 Matches your PCR plate's wells.

 

User Instruction DOWNLOAD

96well
Cellulose

96well
Nylon

 
Cat. No. Item Unit
176-501C Preservation Plate, 96well, Cellulose 5plates/bag
176-502C Preservation Plate, 96well, Nylon 5plates/bag

3well

User Instruction DOWNLOAD

3well
Cellulose

3well
Nylon

To the level 50μL of solution volume per well

The shape to take out easily with tweezers.

 
Cat. No. Item Unit
176-301C Preservation Plate, 3well, Cellulose 10plates/unit
176-302C Preservation Plate, 3well, Nylon 10plates/unit

1well

User Instruction DOWNLOAD

1well
Cellulose

1well
Nylon

To the level 200μL of solution volume per well

 
Cat. No. Item Unit
176-201C Preservation Plate, 1well, Cellulose 10plates/unit
176-202C Preservation Plate, 1well, Nylon 10plates/unit
 

Usage Examples

1.Preservation of oligo-synthesis RNA probe and ISH (ISH:in situ hybridization)

After a week of preservation under normal temperature.

After 4 months of preservation under normal temperature.

There is always a risk of contamination with RNase based on operator error which may influence the outcome of your experiment. Using PVP the samples remain in the same condition for every ISH providing you have preserved samples in a suitable amount for each experiment. The preservation period is more than 4 months under normal temperature.

2.DNA Preservation and Sequence Analysis

Target DNA can be preserved and used for analysis in a later day. PVP with nylon paper chips is recommended for long chain samples like genomic DNA or high value samples. After sufficient drying, is possible to preserve samples for several months. (Preservation period may vary depending on the level of purification.)

 

Preservation Plate has been developed from the study reslut of MEXT's Intellectual Cluster Formation Project <Tokushima Region Noji group (The University of Tokushima)>.

Contact

general WEB catalog
downloadable catalog
downloadable leaflet
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