Preservation Plate-New form of bioresource storage
Watson's Preservation Plate (PVP) has been developed in order to enable storage and transportation of such bioresources
as nucleic acids (DNA / RNA / oligonucleotide) and blood under room temperature and pressure.
Simply place such samples onto paper chips, then dry, the preservation is completed.
It is very compact and space saving in comparison with preserving in tubes samples of liquid condition. Marking space on the plate and compact body make sample storage management easier.
Paper chips can be put directly into the solution to start PCR or in situ hybridization.
Compared to the traditional way of repeated samplings from the same tube, Preservation Plate (PVP) can reduce contamination risk by using different wells on the PVP and / or changing the PVP sheet itself dependent on the sample (Note: Only 96 well PVP can be cut easily with scissors).
Sample deterioration is prevented as the PVP requires no repetition of freezing and melting.
Paper chips do not contain any reagents or salt so it does not choose type of eluent to be used when you recover samples.
Please find a suitable plate type for different bioresourses and sample volumes.
1.Let the paper chip absorb a liquid sample.
* Protective agent is spread on paper chips and it may appear as dot pattern but it does not affect the qualities.
2.Dry the sample.
*Reduced pressure drying is recommended.
Recommended dry time
[96 well] 60 minutes or more
[1/3 well] 90 minutes or more
* Insufficient drying may result in faulty performance.
3.Seal the plate with the protection seal and store it in room temperature.
* Make sure that the film is tightly applied. Loose sealing may cause contamination.
1.Peel off the protection seal and place the paper chip into a container.
Cut out the necessary size of paper chip.
Pick up the paper chip from tweezer hole.
Push the paper chips by tweezers etc.
2.Put the solution into the container and stir.
In the case of agar, rub the paper chip onto agar.
To the level 5 µL of solution volume per well
size: W115mm x D80mm
Material:Polypropylene(plate)
Lower cost per well.
Easy to cut
Matches your PCR plate's wells.
| Cat. No. | Item | Unit | |
|---|---|---|---|
| 176-501C | Preservation Plate, 96 well, Cellulose | 5 plates / bag | |
| 176-502C | Preservation Plate, 96 well, Nylon | 5 plates / bag |
size: W76mm x D26mm
Material:Polystyrene(plate)
To the level 50 µL of solution volume per well
The shape to take out easily with tweezers.
| Cat. No. | Item | Unit | |
|---|---|---|---|
| 176-301C | Preservation Plate, 3 well, Cellulose | 10 plates / unit | |
| 176-302C | Preservation Plate, 3 well, Nylon | 10 plates / unit |
size: W76mm x D26mm
Material:Polystyrene(plate)
To the level 200 µL of solution volume per well
| Cat. No. | Item | Unit | |
|---|---|---|---|
| 176-201C | Preservation Plate, 1 well, Cellulose | 10 plates / unit | |
| 176-202C | Preservation Plate, 1 well, Nylon | 10 plates / unit |
After a week of preservation under normal temperature.
After 4 months of preservation under normal temperature.
There is always a risk of contamination with RNase based on operator error which may influence the outcome of your experiment. Using PVP the samples remain in the same condition for every ISH providing you have preserved samples in a suitable amount for each experiment. The preservation period is more than 4 months under normal temperature.
Target DNA can be preserved and used for analysis in a later day. PVP with nylon paper chips is recommended for long chain samples like genomic DNA or high value samples. After sufficient drying, is possible to preserve samples for several months. (Preservation period may vary depending on the level of purification.)
Preservation Plate has been developed from the study reslut of MEXT's Intellectual Cluster Formation Project <Tokushima Region Noji group (The University of Tokushima)>.
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