Plasma Separation Device "Plasma Filter"

For recovery of microRNA.

Separating plasma from whole blood quickly without cetrifuge.

For simple screening of cancer examination by microRNA.
For transporting microRNA with room temparature.
Applicable for circulating DNA.

Cat. No.	Item	Unit
176-600C	Plasma Filter	5 pieces/ bag

PROTOCOL

Separating plasma from whole blood

Open the lid with 1 claw side

①Open the lid and add 600 µL blood into the central rounded dent. *1

②Blood spreads, leave it for about 5 minutes.

③Remove the plasma separation plate, dry the separated plasma for about 1 hour on the cellulose membrane.

④Sufficiently dried plasma is reservable *2 and transportable with the lid covered.

*1　Assuming that 600 µL whole blood is available, it is possible to recover microRNA which quantity is equivalent of microRNA in 200 µL plasma.
*2　The storage period is approximately 1 to 2 weeks at room temperature depending on setting conditions.

Recovery microRNA from the ingredients

Open the lid of 2 claws

①By opening the lid from 2 claws side, the cellulose membrane containing plasma is easily taken off.
Cut the filter in any size along the perforation.
microRNA can be extracted from the cellulose membrane by the specified elution kit. *3

*3　If applying extraction liquid slightly extra, the microRNA extraction from cellulose membrane may become improved.

The study of microRNA preservation on cancer patients before / after medical treatment

The graph shows the tumor-specific microRNA expression, which measured by ΔΔCT method. The serum value after medical treatment is set as 1.
The expression of microRNA which value was high before treatment decreased after.
The same result was observed by the serum in the cellulose membrane of Plasma Filter.

Note: The study was supported andproceeded by “The Stragetgic Core Technology Advancement Program” programmed by Japanese Ministry of Economy, Trade and Industry in 2011.
（Jointed with Kyoto Prefectural University of Medicine and Ourgenic Co., LTD.）

Product Inquiry

※We also accept inquiries for mass production development of custom shapes, such as OEM and ODM, for plastic products.

Contact