Cell Aggregate Recovery Device “Spheroid Catch”

Trap spheroids simply by filtration!

Size : Max Diameter 30㎜ × 40㎜ H

  • Rapidly screen spheroids that have been cultured in dish.
  • Recover only spheroids larger than 77 µm hole.
  • Only the mesh can be taken off, making it easy to disperse the recovered spheroids.
    Speeding up the passage culture process.

Body: made of Polypropylene
Mesh filter: made of Nylon

Cat. No.ItemUnit
1884-110CSSpheroid Catch20 pieces

PROTOCOL

①Prepare a dish with cultured spheroids.

②Set Spheroid Catch into a 50 mL centrifuge tube.

③Apply the spheroid-cultured medium into Spheroid Catch.

④The mesh catches large spheroids.

⑤Wash mesh filter with PBS(-).

*If containing liquids on the mesh, apply light centrifuge before proceeding to the next step.

⑥Take off Spheroid Catch from a 50 mL centrifuge tube.

⑦Tweezer the edge side of small hole(Not centered)where is on the side of the bottom, then take off the mesh.

⑧Soaking the mesh into the new medium results in easy removal of spheroids.

⑨Use recovered spheroids for test.

This product was jointly developed with the Nojima Laboratory in the Molecular Genetics Research Division (formerly) at Osaka University Institute of Microbial Diseases.
Papers Published in: E Fujibayashi et al. “Isolation of cancer cells with augmented spheroid-forming capability using a novel tool equipped with removable filter” Oncotarget. 2018 Sep 21; 9(74): 33931–33946.

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